The L 4 F 3 Antigen Is Expressed by Unipotent and Multipo tent
نویسنده
چکیده
Antibody 14F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM. CFU-Meg, BFU-E, and CFUMix. In the present study we examined whether cells that do not express the 14F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with l4F3 and complement. The remaining cells generated CFU-GM, BFUE, and CFU-Mix when cultured in the presence of irradiated adherent cell layers from long-term marrow cultures. Marrow cells not expressing the 14F3 antigen. which were
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